Polytene chromosome squash methods for studying transcription and epigenetic chromatin modification in Drosophila using antibodies
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چکیده
منابع مشابه
Polytene chromosome squash methods for studying transcription and epigenetic chromatin modification in Drosophila using antibodies.
The giant polytene chromosomes from Drosophila third instar larval salivary glands provide an important model system for studying the architectural changes in chromatin morphology associated with the process of transcription initiation and elongation. Especially, analysis of the heat shock response has proved useful in correlating chromatin structure remodeling with transcriptional activity. An...
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Drosophila has long been a favorite model system for studying the relationship between chromatin structure and gene regulation due to the cytological advantages provided by the giant salivary gland polytene chromosomes of third instar larvae. In this tissue the chromosomes undergo many rounds of replication in the absence of cell division giving rise to approximately 1000 copies. The DNA remain...
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Drosophila mediopunctata belongs to the tripunctata group, and is one of the commonest Drosophila species collected in some places in Brazil, especially in the winter. A standard map of the polytene chromosomes is presented. The breakpoints of the naturally occurring chromosomal rearrangements are marked on the map. The distribution of breaking points through the chromosomes of D. mediopunctata...
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As outlined in the preceding chapters, an outstanding problem in the field of eukaryotic gene regulation is understanding the biochemical mechanism by which gene-specific activators regulate transcription. In Chapters 11 and 12, we discussed biochemical strategies to dissect how these transcriptional regulators interact with their cognate DNA sequences and enhance transcription from DNA templat...
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ژورنال
عنوان ژورنال: Methods
سال: 2009
ISSN: 1046-2023
DOI: 10.1016/j.ymeth.2009.02.019